High-content analysis of biomarker intensity and distribution in 3D microtissues
نویسندگان
چکیده
Three-dimensional (3D) cell culture methods are widely accepted as more physiologically relevant than conventional 2D cell culture methods and are believed to improve the prediction of drug candidates at an early stage of the drug development process. Here we describe the analysis of a spherical colon cancer microtissue model using the Operetta® High Content Imaging System. In vivo near-infrared (NIR) agents allowed visualization and quantification of cancer-associated biomarker intensity and distribution in microtissues.
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تاریخ انتشار 2012